HI all,
I know this problem has been discussed previously, but I have hit a bump in the road that I am really struggling to get past.
I have successfully selected a particle dataset from 2D and 3D classification, but when I run Refine3D I get the following:
ERROR:
No orientation was found as better than any other.
A particle image was compared to the reference and resulted in all-zero
weights (for all orientations). This should not happen, unless your data
has very special characteristics. This has historically happened for some
lower-precision calculations, but multiple fallbacks have since been
implemented. Please report this error to the relion developers at
I have tried to use the new subset selection tools in Relion 3,0 to exclude particles with outlying statistics, defocus values, CTF figure of merit. To no avail.
I have also tried this on both single and double precision GPU and on CPU only (which failed giving a different error), as well as on Relion 2.1
I found that I could get one iteration to run if I set the reference to not be on absolute grey levels (which it should be as it was made in Relion). On the next iteration it failed though (presumably because now it was expected to be absolute values).
Has anyone come up with a foolproof way of weeding out the duff particles causing this problem?
Thanks,
D.
Dr David Bhella
Director - Scottish Centre for Macromolecular Imaging
MRC-University of Glasgow Centre for Virus Research
Sir Michael Stoker Building
Garscube Campus
464 Bearsden Road
Glasgow G61 1QH
Scotland (UK)
Telephone: 0141-330-3685
Skype: d.bhella
Virus structure group on Facebook: https://www.facebook.com/CVRstructure
Molecular Machines - Images from Virus Research: http://www.molecularmachines.org.uk
CVR website: http://www.cvr.ac.uk
CVR on Facebook: https://www.facebook.com/centreforvirusresearch
[University of Glasgow: The Times Scottish University of the Year 2018]
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