Dear Thomas,
maybe you can try to overlay your drop with LV CryoOil from Jena
Bioscience. Then the evaporation of the solvent is slowed down and the
crystals are directly cryoprotected when you move the crystals through
the oil. We had quite good results when we cryoprotected crystals in
this oil.
Best wishes,
Yvonne
--
Dr. Yvonne Thielmann
Max Planck Institute of Biophysics
Molecular Membrane Biology
Max-von-Laue-Strasse 3
60438 Frankfurt / Main
Germany
Office +49 69 6303 1056
Lab +49 69 6303 1074
Fax +49 69 6303 1002
Am 15.08.2018 um 08:05 schrieb Kajander, Tommi A:
> Yes sorry, i meant paratone-N also.
>
> Tommi
>
> Kohteesta: ferrer
> Lähetetty: keskiviikko 15. elokuuta klo 0.41
> Aihe: Re: [ccp4bb] Fishing crystals from volatile solvent as precipitant
> Vastaanottaja: [log in to unmask]
>
>
> Dear Thomas,
>
> Alternatively you can try shooting on crystals in the drop, in situ. So
> fishing, no cryo. But potentially high radiation damage. Can be
> considered if you have enough crystals, and if your crystallization
> plate makes it possible.
>
> Regards
>
> JL
>
> On 14/08/2018 20:58, Thomas Krey wrote:
> Dear crystallization experts,
>
> We have 3D protein crystals grown from a microseed matrix screening
> vapor diffusion experiment in either
>
> 15% (v/v) Reagent alcohol
> HEPES Na pH 7.5
> 0.2 M MgCl2
>
> or in
>
> 27% Isopropanol
> 0.18 M MgCl2
> 90 mM HEPES Na pH 7.5
> 10% Glycerol
>
> Upon opening the corresponding wells these crystals move quite a bit –
> presumably due to the volatility of the alcohols. Does anyone have a
> good suggestion to stabilize the swirling movements? Does anyone have
> experience, whether these conditions alone can serve as cryo-protectant
> (i.e., do we really have to fish, move into cryo solution and fish again)?
> Any suggestion or input would be highly welcome.
>
> Thank you very much in advance.
>
> Thomas
>
>
> Prof. Dr. Thomas Krey
> Hannover Medical School
> Institute of Virology
> Structural Virology Group
> Carl-Neuberg-Str. 1
> D-30625 Hannover
> phone: +49 (0) 511 - 532 4308
> email: [log in to unmask] <mailto:[log in to unmask]>
>
>
> To unsubscribe from the CCP4BB list, click the following link:
> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
>
> -- -------------------------------- Jean-Luc Ferrer Institut de Biologie
> Structurale 71 Avenue des Martyrs CS 10090 38044 Grenoble Cedex 9 -
> FRANCE Ph.: +33 (0)4 57 42 85 22 Cell: +33 (0)6 89 45 13 57 email:
> [log in to unmask] <mailto:[log in to unmask]>
> --------------------------------
> To unsubscribe from the CCP4BB list, click the following link:
> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
>
>
>
> ------------------------------------------------------------------------
>
> To unsubscribe from the CCP4BB list, click the following link:
> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
>
########################################################################
To unsubscribe from the CCP4BB list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
|