Dear all,
I am writing because I have a problem with 3D auto-refine in Relion 3.0 beta,
When I run an 3D auto-refine job as the instruction in the tutorial,the command followed:
which relion_refine_mpi` --o Refine3D/job022/run --auto_refine --split_random_halves --i Extract/job019/particles.star --ref Class3D/job017/run_it025_class002_box256.mrc --ini_high 50 --dont_combine_weights_via_disc --preread_images --pool 20 --pad 2 --ctf --ctf_corrected_ref --particle_diameter 200 --flatten_solvent --zero_mask --oversampling 1 --healpix_order 2 --auto_local_healpix_order 4 --offset_range 5 --offset_step 2 --sym D2 --low_resol_join_halves 40 --norm --scale --j 2 --gpu
I get the following error message when the job is running in every iteration:
WARNING: FSC dipped below 0.5 and rose again. Using higher resolution of 12.744 A, instead of 17.7 A.
This is not necessarily a bad thing. Often it is caused by too tight masks.
WARNING: FSC dipped below 0.5 and rose again. Using higher resolution of 12.744 A, instead of 17.7 A.
This is not necessarily a bad thing. Often it is caused by too tight masks.
WARNING: FSC dipped below 0.5 and rose again. Using higher resolution of 12.744 A, instead of 16.7684 A.
This is not necessarily a bad thing. Often it is caused by too tight masks.....
The job runs correctly, just has this error message, is it because the mask diameter is too small or some else problem ?
Can anyone help me?
Many thanks in advance.
Best regards,
Lifei
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