Dear all,
I am trying to generate a power spectrum from a negatively stained micrograph, which contains the filament I am interested. However, the micrograph itself contains the bacteria and my filaments. I only want to generate the power spectrum of my filaments but exclude bacteria or other non-useful information. I have tried boxing the filament out in EMAN2 and generate power spectrum, but did not get any layer line or anything similar to a power spectrum of a helix. I am wondering if there is a better way to do this?
Best,
Yangqi
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