Dear all,
I am trying to process my EM micrographs. They are .tif images (Not a stacked movie, just a single image). And I tried using EMAN2 to convert them to .mrc using the command e2proc2d.py, however, it raised the following errors:
raceback (most recent call last):
File "/usr/local/bin/e2evalimage.py", line 1072, in <module>
main()
File "/usr/local/bin/e2evalimage.py", line 98, in main
gui=GUIEvalImage(args,options.voltage,options.apix,options.cs,options.ac,options.box,options.usefoldername,options.constbfactor,options.astigmatism)
File "/usr/local/bin/e2evalimage.py", line 166, in __init__
n=EMUtil.get_image_count(i)
File "/usr/local/lib/EMAN2db.py", line 539, in db_get_image_count
raise Exception,fsp
Exception: micrographs/WT_DV-001.tif
I am wondering if anyone had any experience how to deal with this? Or can I just directly use .tif images?
Best,
Yangqi
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