Hi Sjors,
Thank you! The new_box trick has helped and Auto-picking seems to be running well.
Cheers,
Jeongyoon
> On 18 Jul 2018, at 12:04, Sjors Scheres <[log in to unmask]> wrote:
>
> Hi Jeongyoon,
>
> That's strange! Do the images still look OK?
>
> relion_image_handler --new_box 4096 might do the trick of setting them
> all back in the same box.
>
> HTH, S
>
>
>
> On 07/18/2018 10:58 AM, Jeongyoon Choi wrote:
>> Dear all,
>>
>> Thanks a lot.
>> Apparently, for whatever reason, each image has slightly different number of pixels (varing from 4089 to 4096).
>> Is there a clipping or cropping function in Relion to make all images in the same number of pixels?
>>
>> Best wishes,
>> Jeongyoon
>>
>>
>>
>>
>>
>>
>>> On 18 Jul 2018, at 07:57, Sjors Scheres <[log in to unmask]> wrote:
>>>
>>> Hi Jeongyoon,
>>> Perhaps you could try to run relion_image_handler --stats on the
>>> micrographs.star file (you may need to change the rlnMicrographName label
>>> into rlnImageName for this). Just to make sure there is nothing unexpected
>>> there..
>>> HTH,
>>> Sjors
>>>
>>>> Dear all,
>>>>
>>>> I’m trying to perform auto-picking of 100 micrographs containing 4096
>>>> pixels on each side and all are in the same magnification.
>>>> I’m sure the images are in the same size, but when I ran the
>>>> auto-picking I got these errors as attached here.
>>>> Does anyone get the same problem as me? Or anyone could help me in this
>>>> please?
>>>> It seems the program tries to change the pixel size for whatever reason??
>>>>
>>>> Thanks a lot,
>>>> Jeongyoon
>>>>
>>>> [cid:7A1B2F1B-D0D4-4F94-BE39-E11A885E5595@bioch.ox.ac.uk]
>>>>
>>>> ########################################################################
>>>>
>>>> To unsubscribe from the CCPEM list, click the following link:
>>>> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1
>>>>
>>>
>>> --
>>> Sjors Scheres
>>> MRC Laboratory of Molecular Biology
>>> Francis Crick Avenue, Cambridge Biomedical Campus
>>> Cambridge CB2 0QH, U.K.
>>> tel: +44 (0)1223 267061
>>> http://www2.mrc-lmb.cam.ac.uk/groups/scheres
>>>
>
> --
> Sjors Scheres
> MRC Laboratory of Molecular Biology
> Francis Crick Avenue, Cambridge Biomedical Campus
> Cambridge CB2 0QH, U.K.
> tel: +44 (0)1223 267061
> http://www2.mrc-lmb.cam.ac.uk/groups/scheres
>
########################################################################
To unsubscribe from the CCPEM list, click the following link:
https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCPEM&A=1
|