Hi Jeongyoon,
That's strange! Do the images still look OK?
relion_image_handler --new_box 4096 might do the trick of setting them
all back in the same box.
HTH, S
On 07/18/2018 10:58 AM, Jeongyoon Choi wrote:
> Dear all,
>
> Thanks a lot.
> Apparently, for whatever reason, each image has slightly different number of pixels (varing from 4089 to 4096).
> Is there a clipping or cropping function in Relion to make all images in the same number of pixels?
>
> Best wishes,
> Jeongyoon
>
>
>
>
>
>
>> On 18 Jul 2018, at 07:57, Sjors Scheres <[log in to unmask]> wrote:
>>
>> Hi Jeongyoon,
>> Perhaps you could try to run relion_image_handler --stats on the
>> micrographs.star file (you may need to change the rlnMicrographName label
>> into rlnImageName for this). Just to make sure there is nothing unexpected
>> there..
>> HTH,
>> Sjors
>>
>>> Dear all,
>>>
>>> I’m trying to perform auto-picking of 100 micrographs containing 4096
>>> pixels on each side and all are in the same magnification.
>>> I’m sure the images are in the same size, but when I ran the
>>> auto-picking I got these errors as attached here.
>>> Does anyone get the same problem as me? Or anyone could help me in this
>>> please?
>>> It seems the program tries to change the pixel size for whatever reason??
>>>
>>> Thanks a lot,
>>> Jeongyoon
>>>
>>> [cid:7A1B2F1B-D0D4-4F94-BE39-E11A885E5595@bioch.ox.ac.uk]
>>>
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>>
>> --
>> Sjors Scheres
>> MRC Laboratory of Molecular Biology
>> Francis Crick Avenue, Cambridge Biomedical Campus
>> Cambridge CB2 0QH, U.K.
>> tel: +44 (0)1223 267061
>> http://www2.mrc-lmb.cam.ac.uk/groups/scheres
>>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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