After some searching, I couldn't find an answer to this question.
Up until recently I had always seen 2D classes similar to looking at different angles of a solid object (like I was rotating the protein in my hand). I see a surface. This is different from a 2D projection, which I have always seen as 3D density information flattened into a 2D image. In a projection, features have no context in the z-axis. So if you see something, you wouldn't be able to tell its absolute position on the particle, only its relative orientation.
I had always seen classes as the opposite: if you see features in a 2D class, it is on that face of the particle.
Is that true? Or do I have my understanding mixed up? Recently some of our classes (Relion) have shown features we expected on a different face (based on biochemical data).
Thank you for indulging a lowly assistant :)
- Austin Dixon
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