Dear all,
I am trying to join 3 particles.star files from previous Class2D and run Class2D again but keep getting the error message below.
I tried to figure out which dataset has problem by joining different sets and run Class2D,
SetA + SetB --> no error;
SetB + SetC --> no error;
SetA + SetC --> no error;
But as long as I added the third set, it failed.
I also tried to join all the micrographs and extract particles again with "Yes" to "Normalized particles", but still get the same error message when I run Class2D.
Did I miss something? Any suggestions are welcome.
Many thanks,
Candy Tang
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fn_img= [log in to unmask] bg_avg= 1.93523e-314 bg_stddev= 0 bg_radius= 145.349
fn_img= [log in to unmask] bg_avg= 1.93523e-314 bg_stddev= 0 bg_radius= 145.349
ERROR:
ERROR: It appears that these images have not been normalised to an average background value of 0 and a stddev value of 1.
Note that the average and stddev values for the background are calculated:
(1) for single particles: outside a circle with the particle diameter
(2) for helical segments: outside a cylinder (tube) with the helical tube diameter
You can use the relion_preprocess program to normalise your images
If you are sure you have normalised the images correctly (also see the RELION Wiki), you can switch off this error message using the --dont_check_norm command line option
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