Hi,
Yes, for long-term 'cold' storage, (p)bzip2 can be better.
The advantage of TIFF for processing is that it is being supported
by many programs (MotionCor2, cisTEM, RELION 3, etc) and
compression/decompression is handled transparently. Otherwise, users
have to decompress huge movies before processing.
In RELION 3, our new motion refinement directly reads raw movie frames
(in MRCS or TIFF). You no longer have to write aligned movies and
movie particles. So if you record images in TIFF from the beginning,
you can save lot of storage.
Best regards,
Takanori Nakane
On 2018/06/03 14:15, Oliver Clarke wrote:
> I would vote for bzip2 over compressed tiff.
>
> For a test 50 frame, 1.4G stack, pbzip2 gives a 214M archive, while mrc2tif gives a 264M tiff - and pbzip2 is much faster to compress/decompress at least on my system (GPU workstation with 24 cores/48 threads)
>
> Cheers
> Oli
>
>> On Jun 3, 2018, at 9:00 AM, Dimitry Tegunov <[log in to unmask]> wrote:
>>
>> Hey!
>>
>> In addition to what Takanori wrote: If you're stuck with EPU for data collection, this tool can run on the K2 computer to convert gain-uncorrected MRCs to compressed TIFFs on-the-fly: https://github.com/dtegunov/stacker2. It will also happily compress your old files, assuming they contain only integers.
>>
>> I very much hope that one day we will get an EPU version with TIFF support for K2/3 data. And with most of SerialEM's functionality. And a pony. In this exact order, please ;-)
>>
>> Cheers,
>> Dimitry
>>
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