Hi all,
I am using Relion 2.1 to do a helical refinement. I am using following parameters for the helical refinement:
--healpix_order 3 \
--auto_local_healpix_order 4 \
--offset_range 5 \
--offset_step 2 \
--sym C1 \
--low_resol_join_halves 40 \
--norm \
--scale \
--helix \
--helical_outer_diameter 60 \
--helical_nr_asu 2 \
--helical_twist_initial 105 \
--helical_rise_initial 10 \
--helical_z_percentage 0.1 \
--helical_symmetry_search \
--helical_twist_min 100 \
--helical_twist_max 120 \
--helical_twist_inistep 0.02 \
--helical_rise_min 5 \
--helical_rise_max 20 \
--helical_rise_inistep 0.01 \
--sigma_tilt 5 \
But some how, I had issue with the z percentage 0.1. When I did refinement, it said "Z percentage is out of range under current settings!". I wanted to know which parameters violate the setting rules. Could you help me out?
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