How do your twinning statistics look? It could be that the real space group has lower symmetry (and thus more space for your G4 DNA).
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Phoebe A. Rice
Dept. of Biochem & Mol. Biol. and
Committee on Microbiology
https://voices.uchicago.edu/phoebericelab/
On 4/20/18, 8:31 AM, "CCP4 bulletin board on behalf of Rafal Dolot" <[log in to unmask] on behalf of [log in to unmask]> wrote:
Dear CCP4BB,
I've recently collected data for 11mer build of DNA (9xG, 2xT). XDS, and
DIALS gave me similar solution - SG: I2(1)2(1)2(1) or I222, with cell
dimension 20.65, 22.96, 43.37, 90, 90, 90, what is too small for this
size of the molecule. 11mer is rich in G, so we expect the G-tetraplex
formation. Data were collected to almost 1 A, so it should be enough for
trials with direct methods/ab initio solution. What I should do first to
find correct SG and/or cell parameters?
Best regards,
Rafal
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|Rafal Dolot, Ph.D. |
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|Polish Academy of Sciences |
|Centre of Molecular and Macromolecular Studies|
|Department of Bioorganic Chemistry |
|Macromolecular Crystallography Team |
|Sienkiewicza 112 |
|90-363 Lodz, Poland |
|Phone: +48(42)6803215 |
|Cell: +48 502897781 |
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