Dear Steven,
I would recommend that you closely follow the tutorial available on the
Relion Wiki first. This will guide you through the most common steps and
will hopefully provide you with insights on how to solve this problem.
HTH,
Sjors
> Dear all,
>
>
>
> I have some single particle cryoEM .mrc files imported to Relion. When I
> was trying to do the motion correction using MotionCor2 inside relion,
> most of the commands auto-generated by relion are incomplete because there
> is no input file specified after “-InMrc”. I tried Relion 2.1b1 and
> 2.1, all having the same issue. By manually adding the input files to the
> command, I could make MotionCor2 work correctly. Is there any special
> parameter required for working with mrc files?
>
>
>
> Also, I specified the first frame to be 1 and the last frame to be 0,
> since I am not sure how many frames each mrc file has. Is that okay?
> Thanks.
>
>
> Steven
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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