Hi CCPEM
I'm sorry to bring this discussion up again as in the link
https://www.jiscmail.ac.uk/cgi-bin/webadmin?A2=CCPEM;2b838c96.1802
and yes, I definitely wholeheartedly agree with Bjorn's excellent points.And I also agree that the ideal case would be extracting a single particle per box. But in a sample where there are crowded particles would changing the diameter of the background circle during extraction be a better way than extracting with a bigger box and masking out only the single particle of interest. in the 2D classification step?
Sincerely
Joshua Lobo
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