Hi,
It is fine to work with the original image orientation for DTIFIT (we would generally recommend this) and then reorient/transform/resample the resulting images.
Unless you want the information in a different space (e.g., to match an anatomical scan or the MNI template) then you don't have to do any spatial transformations if you do not want to. If you do want the information in a different space then we would recommend registration between a diffusion image and an image in the other space and then apply that transformation to your diffusion results (FA, MD, etc).
All the best,
Mark
> On 2 Jan 2018, at 15:04, Surya Rajan <[log in to unmask]> wrote:
>
> Hi,
>
> I had a question regarding the scalar maps. I have a 2D acquired axial DTI image which means if it is reconstructed to sagittal or coronal planes, some of the information is lost and any measurement/computation I do in these reconstructed planes is "less correct" than those done in the original plane.
>
> If I first do DTIFIT on the original axial DTI to create the FA, MD maps and then reconstruct these maps to the other planes - is this better (since the scalar maps are constructed using directional information)?
>
> The aim is to make sagittal FA, MD measurements on an axial DTI scan.
>
> Thanks,
> Surya
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