Hi David,
We usually set the min inter-particle distance to 50-60% of the particle
diameter. Then we change the threshold to get more/less particles.
HTH,
Sjors
On 01/10/2018 11:24 AM, David Bhella wrote:
> Hi
>
> I am autopicking some large virus particles (using a binned reference) and having some problems with the minimum inter-particle distance threshold. I find that if I set this above 568 angstroms I get zero particles picked.
> Set to 568 I get lots picked (with a low threshold), set to 569 or above I get none (regardless of any other settings).
>
> Any suggestions would be appreciated.
>
> Thanks,
> D.
>
>
> Sent from my iPad
>
> [University of Glasgow: The Times Scottish University of the Year 2018]
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
|