Reza Khayat, PhD
Assistant Professor
City College of New York
Department of Chemistry
New York, NY 10031
________________________________________
From: Collaborative Computational Project in Electron cryo-Microscopy <[log in to unmask]> on behalf of Sjors Scheres <[log in to unmask]>
Sent: Wednesday, January 10, 2018 4:32 AM
To: [log in to unmask]
Subject: Re: [ccpem] maxsig value for Classifying out Junk particles
Hi Joshua,
You may also want to consider splitting the data set in batches of say
100k particles for junk removal by class2D. There are some memory
problems with large STAR files on some systems. The next release will
stop these from happening.
HTH,
Sjors
On 01/09/2018 10:16 PM, Joshua Lobo wrote:
> Hi CCPEM
>
> I had a question regarding the --maxsig value for 2D Classification with the overall goal of weeding out junk particles and keeping iteration time down. Is it advisable to use the maxsig value when for the initial classification steps? I have a large dataset that I would like to classify ( greater than 1Million Particles) . I have binned the data to increase speed already. and am using the new subsets during first iterations mechanism in relion2.1
>
>
> Any suggestions are welcome
>
> Sincerely
> Joshua Lobo
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
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