Hi Simona,
If you don't care about CTF correction, then just switch it off in
Tanmay's scripts. Then whether the data is phase-plate or not no longer
matters.
HTH,
Sjors
On 01/12/2018 02:00 PM, Simona Sorrentino wrote:
> Dear all,
> I acquired a dataset with the Volta Phase Plate, and I would like to do subtomogram averaging from this data, without CTF correction, in RELION. Are there any suggestions on how to prepare the 3D CTF models, for Phase Plate data?
> Thanks in advance
>
> Simona
>
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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