Hi CCPEM
I had a question regarding the --maxsig value for 2D Classification with the overall goal of weeding out junk particles and keeping iteration time down. Is it advisable to use the maxsig value when for the initial classification steps? I have a large dataset that I would like to classify ( greater than 1Million Particles) . I have binned the data to increase speed already. and am using the new subsets during first iterations mechanism in relion2.1
Any suggestions are welcome
Sincerely
Joshua Lobo
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