Dear Rick,
If you've done CTF correction, and you thus have defocus values etc in
the STAR file, then you should use --ctf in the subtraction too. If this
gives an error, your star file is somehow corrupt: did you manually
change it?
If you've collected 'in-focus' data and you have thus skipped the CTF
estimation, then you can skip it in the subtraction too.
HTH,
Sjors
On 01/19/2018 12:24 AM, Rick Baker wrote:
> Hello CCPEM,
>
> I tried to use the signal subtraction function in Relion2 and got the
> following error:
>
> CTF::initialise: ERROR: CTF initialises to all-zero values. Was a correct
> STAR file provided?
> File: /data/software/src/relion/relion2/src/ctf.cpp line: 188
>
> If I turn off the CTF flag, I don't get the error, and I get a subtracted
> particle stack.
>
> I am using phase-shifted data. Is this the cause of the error? Can this
> module in Relion handle phase shifts? Is there something else that may be
> the problem? I have followed the protocol outlined in the MM of Bai, et al.
> 2015, using the data.star file and map from a 3D auto-refine run.
>
> Thanks,
> Rick
>
>
>
>
> ------------------------------------------------------------------
> Richard Baker, Ph.D.
> Damon Runyon Post-Doctoral Fellow
> Leschziner Research Group
> Department of Cellular and Molecular Medicine
> University of California, San Diego
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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