Hi all,
Does the process (as described in this email thread for relion1.4) of:
- restricting the angular search around prior values by changing _rlnOrigin[X/Y/Z] to _rlnOrigin[X/Y/Z]Prior and _rlnAngle[Rot/Tilt/Psi] to _rlnAngle[Rot/Tilt/Psi]Prior in the .star file
- restricting the angular range sampled by --sigma_ang XX
- setting "initial angular sampling" to the same value as "local searches from auto-sampling"
in order to perform local searches from the outset around the prior values, also apply to relion 2.1?
Many thanks,
James
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