Dear all
I have a somewhat conceptual problem.
My sample corresponds to a heterodimer, but I had some difficulties in separating and reconstituting the dimer from labelled/unlabelled subunits. I got one reference sample, for which I registered a TROSY experiment from which I can guess whether a peak belongs to chain A or B.
So far I have entered the sequence information, 1 MolSystem, 2 molecules with associated chains A and B.
I have a series of backbone experiments acquired on a uniformly labelled sample, I have a TROSY experiment from this sample and another one on a sample with just one chain labelled (but I do NOT have the corresponding set of triple resonance experiments because the sample concentration was too low).
Now, what is the best way to get my root resonances and proceed with the semi-automatic assignment ? It would be nice to be able to flag from the beginning, to which chain a spin system belongs. But I did not find any option. Is there a way to do this ?
Otherwise I doubt that I can initiate root resonances from two different peak lists (I could create two lists from the beginning) and working with two projects from the beginning does not seem possible as I should be able to treat the whole information together in order to be able to change the association of a spin system with a given chain during the assignment process.
Any suggestion is welcome !
Thanks in advance
Beate
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