Hi All,
We have just had RELION installed, and I was trying to run the betagal tutorial example. However, I ran into a glitch almost right away. The motion correct did not run. "run.err" file has the following message for each of the movie files (of which there are 15 in contrast to 16 mentioned in the tutorial):
"
WARNING: there was an error executing: /tools/apps/Crystallography/RELION/bin/relion_run_motioncorr -InMrc /lscratch/relion21_tutorial/betagal/Micrographs/Falcon_2012_06_12-14_33_35_0_movie.mrcs -OutMrc MotionCorr/job012//lscratch/relion21_tutorial/betagal/Micrographs/Falcon_2012_06_12-14_33_35_0.mrc -Bft 150 -PixSize 3.54 -OutStack 1 -Patch 5 5 -Trunc 1 -Kv 300 -FmDose 1 -InitDose 0 -Gpu 1 >> MotionCorr/job012//lscratch/relion21_tutorial/betagal/Micrographs/Falcon_2012_06_12-14_33_35_0.out 2>> MotionCorr/job012//lscratch/relion21_tutorial/betagal/Micrographs/Falcon_2012_06_12-14_33_35_0.err
"
Followed by the final line:
"
GPL Ghostscript 9.07: Unrecoverable error, exit code 1
"
Following is the Falcon_2012_06_12-14_33_35_0.err file:
"
The following warnings were encountered upon command-line parsing:
WARNING: Option -InMrc is not a valid RELION argument
WARNING: Option -OutMrc is not a valid RELION argument
WARNING: Option -Bft is not a valid RELION argument
WARNING: Option -PixSize is not a valid RELION argument
WARNING: Option -OutStack is not a valid RELION argument
WARNING: Option -Patch is not a valid RELION argument
WARNING: Option -Trunc is not a valid RELION argument
WARNING: Option -Kv is not a valid RELION argument
WARNING: Option -FmDose is not a valid RELION argument
WARNING: Option -InitDose is not a valid RELION argument
WARNING: Option -Gpu is not a valid RELION argument
The following errors were encountered upon command-line parsing:
ERROR: Argument --i not found or invalid argument
ERROR:
Errors encountered on the command line (see above), exiting...
File: /tmp/relion/src/motioncorr_runner.cpp line: 73
"
Can somebody guide me how I can resolve this?
Thanks,
Ketan Gajiwala
|