I've tried multiple masks with multiple masking settings in an attempt to get proper post-processing done on our structure, but to no avail. I've tried harsher lowpass filtering, more pixels of map extension/soft-edge, and looser initial binary thresholds. I am using the correct MTF for our detector. I have looked at each map I send to post-processing and they all look fine.
I wonder if perhaps post-processing is struggling since there is a prominent feature jutting out of the protein that it thinks is noise?
Would love any guidance on this!
Thank you,
Austin Dixon
Rockefeller University
O'Donnell Lab
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