Any chances for some guadinium leftover from a refolding protocol?
See second line in fig 1 here, quite similar stacking: http://www.mdpi.com/1420-3049/20/5/9214/htm
Best,
F.
Federico Forneris, PhD
----
The Armenise-Harvard Laboratory of Structural Biology
Dept. Biology and Biotechnology
University of Pavia
Via Ferrata, 9
I-27100 Pavia - ITALY
http://fornerislab.unipv.it
-----Original Message-----
From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of Stephen Cusack
Sent: lunedì 9 ottobre 2017 17:40
To: [log in to unmask]
Subject: [ccp4bb] help identifying unknown density
Dear All,
�� I am refining the crystal structure of an E. coli expressed protein
at 2.65 A resolution. The crystals grew in
0.1 M MES pH6, 0.7 M sodium formate pH 6. Ni-NTA was used in the
purification.
There are four molecules in the asymmetric unit. A particular histidine
in each molecular has clear, strong extra density which looks like a
metal bonded to an imidazole nitrogen (distance approx 2 A) with three
other co-ordinating atoms in a triangular planar arrangement (see
attached screen shot for unbiased extra density, atoms are only put in
the density for illustrative purposes).
Has anyone seen anything like this ? Any suggestions ?
thanks very much
Stephen
--
**********************************************************************
Dr. Stephen Cusack, FRS
Head of Grenoble Outstation of the European Molecular Biology Laboratory (EMBL)
Group leader in structural biology of protein-RNA complexes and viral proteins
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