Hi John,
I just had this problem appearing for no apparent reason, since the header said that I was between 0 and 1. Running relion_image_handler with the threshold_above and threshold_below options on that mask solved the problem.
Perhaps this works for you too.
Cheers,
Michael
> Am 04.09.2017 um 10:09 schrieb Sjors Scheres <[log in to unmask]>:
>
> Hi John,
> What does the following report?
> relion_image_handler --stats --i mask.mrc
> S
>
>> On 09/02/2017 04:23 PM, John Heumann wrote:
>> When trying to do a 3D refinement with a solvent mask using Relion 2.1 downloaded and built August 17 , I'm getting an error at the end of the 1st iteration:
>>
>> MlOptimiser::solventFlatten: ERROR solvent mask should contain values between 0 and 1 only...
>>
>> That seems straightforward enough, but as far as I can tell the mask values akready are all between 0 and 1. E.g. IMOD reports:
>>
>> Minimum density ....................... 0.0000
>> Maximum density ....................... 1.0000
>> Mean density .......................... 0.18984
>>
>> The mask is a mode 2 (32-bit real) file.
>>
>> Any suggestions?
>>
>> Regards,
>> -jh-
>
> --
> Sjors Scheres
> MRC Laboratory of Molecular Biology
> Francis Crick Avenue, Cambridge Biomedical Campus
> Cambridge CB2 0QH, U.K.
> tel: +44 (0)1223 267061
> http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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