Hi Clara,
If you did normalise the images, you can ignore this and run with the
additional argument: --dont_check_norm
HTH,
Sjors
On 09/27/2017 08:22 PM, Clara Gilda Altomare wrote:
> Dear all,
>
> I'm a second year PhD student in a single particle cryo-EM lab.
>
> When trying to use a subset selection of subtracted particles in 3D
> classification I ran into the following error message:
>
> ERROR: It appears that these images have not been normalised to an average
> background value of 0 and a stddev value of 1.
>
> When using the full subtracted stack 3D classification runs without issue.
> Has anyone seen this error or know why it is happening?
>
>
> Thanks,
> Clara
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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