Hi Simona,
You could use a very tight sigma_rot and sigma_tilt. Use a 3D
classification job, with a single reference, and a sufficiently fine
angular sampling for this.
HTH,
Sjors
On 08/03/2017 08:25 AM, Simona Sorrentino wrote:
> Dear all,
>
> I have reasonably well aligned particles from a 3D refine job. I would like to improve the alignment restricting the angular search only to the psi angle.
> Is it possible to do it in relion2?
>
> Thanks and all the best
>
> Simona Sorrentino
> --------------------------------------
> PhD Student
> Medalia Group
> Department of Biochemistry
> University of Zurich
> Winterthurerstr. 190
> CH-8057 Zurich
>
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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