Hi!
I have been testing that is it possible to use some unusual experiments in Analysis 2.4.2. (like hacaCONcaHA). Unfortunately, I have not been able to make my own experiment prototypes. This leads to problems in the “Pick & Assign From Roots” and the “Protein Sequence Assignment” windows.
In “Pick & Assign From Roots”, I can’t assign root resonances. Then I noticed that I can fix the problem by using large tolerance in setup “Root 1H Dim 1”. I guess this is one way to fix this. However, I am not sure why I have this problem.
In “Protein Sequence Assignment”, the problem is that I cannot get my spectrum to show up in “Query Windows and Spectra” and “Match Windows and Spectra” (Of course, I need to use it only in one table by clicking “Yes” to “Use?” column.). So, I cannot start using Spin System Table tab.
Could I somehow use “Pick & Assign From Roots” and “Protein Sequence Assignment” without filling “Full Type” column in Experiment Types tab in Experiments window? I have also been wondering, if I would just select some “Full Type” which seems to work. I am not sure how Analysis works, so it might lead to problems?
Mikael Karjalainen
Doctoral student
Permi NMR Lab, Department of Chemistry
University of Jyväskylä, FINLAND
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