Hi Joel,
You could try to ignore the CTFs until their first peak (on the CTF tab).
HTH,
Sjors
On 07/19/2017 02:33 PM, Joel Meyerson wrote:
> Hi,
> I'm working with a 170 kDa membrane protein imaged on a Krios/K2. In
> the micrographs the particles have the expected features, and they
> adopt multiple orientations.
>
> I manually picked ~12,000 particles and tried 2D classification in
> Relion 2.1, but the particles seem to collapse into just a few classes
> with poorly defined features. I tested a range of mask diameters, and
> also tried limiting the expectation step resolution to 10 A, but the
> classes did not improve.
>
> Are there other tips out there for working with smaller proteins?
>
> Thanks,
> Joel
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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