Hello everybody,
I was looking for suggestion on the best way to identify and refine carbohydrates in a protein. This is the scenario:
- I have 10 molecules in the biological assembly
- I used sf9 expression system, so I have random glycolisation and types of glycolisations. I have some hint about possible glycolisation sites
- I believe I have only 1 glysolisation per monomer
- I have 2 molecule per AU. Space group C2
I would like (even if I have to write down a script for it) to track this glycolisation, identify the most probable ones, determine the restrictions for each and refine them. I could do it by hand on every refinement cycle (or a couple of cycles), but for many datasets it would take ages.
What protocol would you suggest?
I really appreciate any help in this subject.
Regards,
Gustavo
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