Dear all,
What is the purpose of the --premultiply_ctf option for particle extraction with relion_preprocess?
The help (and the code) is clear - it multiplies the micrograph by the CTF specified in the .star file before extraction, but what would be the purpose of doing this?
Is this intended as a way to obtain a CTF-corrected particle stack? I’m confused because correction should require dividing by the CTF (or using a Wiener filter, etc).
Thank you,
David
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