Dear all,
I am running Class3D (without alignment) on subtracted particles
by using a volume cut out from the original volume as a model and a mask
masking the remainder. Everything seems fine and I can view the
resulting mrc files in 'File Display' and in Chimera. However, when
viewing the run_itxxx_model.star files in 'File - Display' I just get
uniformly gray images. I have tried several values for the parameters
Sigma Contrast, Black value and White Value without seeing a change. I
suppose that it has to do with incorrect normalization or very low
signals but I am wondering whether this is the expected behavior.
Thanks, Dieter
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Dieter Blaas,
Max F. Perutz Laboratories
Medical University of Vienna,
Inst. Med. Biochem., Vienna Biocenter (VBC),
Dr. Bohr Gasse 9/3,
A-1030 Vienna, Austria,
Tel: 0043 1 4277 61630,
Fax: 0043 1 4277 9616,
e-mail: [log in to unmask]
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