Hi Ricardo,
The sampling in the local resolution estimation is not taking the
symmetry into account. Because it will sample the local resolution on a
regular grid, the resulting map will indeed not obey strict symmetry.
Hopefully, it is still useful. Re-enforcing the symmetry with the
image_handler may indeed help. Overall, the results should be similar.
HTH,
Sjors
On 06/02/2017 09:29 PM, Ricardo Righetto wrote:
> Hi,
>
> I am using the --locres option in relion_postprocess and would like to
> understand how can I ensure a sampling compatible with the symmetry of my
> particle. The problem is, I have a symmetrical particle but the results of
> my local-resolution evaluation are not symmetrical.
>
> In this particular case, my box is 100^3 pixels and pixel size is 1.3 A. I
> set --locres_sampling to 26 A, and the local resolution is uniformly
> sampled within the box as expected, but the resolution values in the
> _locres.mrc map do not follow the symmetry of my particle (Cn), which I
> would expect.
>
> Am I missing something here?
>
> I thought of (re-)enforcing symmetry afterwards with relion_image_handler
> on the _locres.mrc and _locres_filtered.mrc maps, but not sure if that's a
> good solution.
>
> Thanks in advance!
>
> Best,
>
>
> --
> Ricardo Diogo Righetto
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
|