Dear all,
I have a star file with orientations for a data set of helical particles, coming from a successful relion_refine run using parameters adapted to helical symmetry.
However, when I try to use relion_reconstruct, and use the parameters for helical symmetry, I get an aberrant reconstruction as output. It follows the symmetry, but densities are aberrant and stretched in z. If I use the exact same star file for relion_reconstruct without helical parameters, the resulting map is perfectly fine.
So, this command does not work :
relion_reconstruct --i orientations.star --o map.mrc --angpix 1.632 --nr_helical_asu 8 --helical_twist -29.2 --helical_rise 3.9 --ctf --j 6
And this command works :
relion_reconstruct --i orientations.star --o map.mrc --angpix 1.632 --ctf --j 6
Has anyone experienced the same problem ?
Thanks,
Best wishes
Ambroise
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