Hi all,
I have recently been trying to run local CTF estimation with gCTF, but I have been getting strange results. I have ran global CTF estimation for all micrographs in my micrograph folder with gCTF, and have automatically picked particles for all of them with gAutomatch. When I try ro run gCTF for local CTF estimation, it seems to detect properly all the micrographs and coordinate files and it works fine for the first micrograph, producing for each particle reasonable U and V defocus values and CCC values. However, from the 2nd micrograph on, it assigns to all particles in the same micrograph the global defocus values previously calculated with global CTF correction and outputs “nan” for the CCC value of all particles (except the first one on each micrograph). I am running this locally on a GTX 1070, and have tried running on a GTX 1080 as well but got similar results.
I was wondering if anyone has encountered similar problems?
Thanks a lot in advance
Best wishes,
Rafa
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