Hi Ahmed,
Yes, you can do this. However, subtracting the detergent belt will
probably be difficult, as you don't have a good model for it. But you
can try.
HTH,
Sjors
On 05/03/2017 10:52 PM, Ahmed-Noor Adam Agip wrote:
> Hi,
>
>
> I understand particle subtracted experimental images in relion can aid in 3D classification, but is it valid to use the very same subtracted experimental images to refine the structure? For example, if I plan to subtract away part of the detergent belt of my protein, in order to align my protein better during 3D refinement?
>
>
> Best wishes,
> Ahmed
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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