Dear H.Sin,
These references may help you:
Meireles, M., Aimar, P., and Sanchez, V. (2004) Albumin denaturation during
ultrafiltration: effects of operating conditions and consequences on membrane
fouling, Biotech. Bioeng. 38, 528-534.
Schratter, P., (2004) Purification and concentration by ultrafiltration, Meth. Mol.Biol.: Protein Purification Protocols 244, 101-116
Sweryda-Krawiec, B., Devaraj, H., Jacob, G., and Hickman, J. J. (2004) A new
interpretation of serum albumin surface passivation, Langmuir 20, 2054-2056.
Adding 1% BSA to your buffer and pre-treating the centricon diafiltration membrane does prevent aggregation and improves protein yield by several fold.
Additionally, adding 50 mM L-Arginine (pH’ed to buffer) prevented aggregation and produced nice protein crystals.
Best wishes,
Avinash
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