If your signal goes all the way to the half Nyquist frequency, oversample your map to have the pixel size equal to 1/3rd of the highest resolution. Your map will look much better in coot. Real space fitting will work much better as well.
Best,
Petr
> On May 17, 2017, at 11:24 AM, MyeongSeon Lee <[log in to unmask]> wrote:
>
> Hi, all.
>
> I have collected cryoEM data and want to use Coot and CCP4 program to build model and to refine it.
>
> 1. What is the steps to do this?
> 2. How do I convert cryoEM map file to MTZ file?
> 3. Can I also use Phenix for this purpose?
>
> Thanks to all for your help in advance.
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