Hello everyone!
i want phasing my crystal by heavy atom derivazation, before x-ray experiment i did native page to confirmation which heavy atom may bind to my protein. the attachment is the native page picture, but the protein is hard to enter in the gel, my protein pI 5.5, the electrophoresis buffer ph7.5, and run at 60mA for 1h. can anyone give me a suggestion to adust the experiment? or estimate which heavy atom bind to my protein from the gel picture?
sincerely
Liuqing chen
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