some correction on my previous message:
one image took 44.74 hour for auto-picking,
two images took 89.48 hour for auto-picking.
MPI=1
RAM=64Gb
around 600 particles pre image
xue
On 2017-02-01 11:22, X.Y. Pei wrote:
> Dear CryoEM community:
> I have a job on the cluster with MPI=1, 64Gb RAM for 44.7 hour, auto
> picking from two images (around 600 particles per image) in order to
> optimize threshold and Dmin took 89.3hour. Here is my setting:
>
> application='`which relion_autopick` --i 2mics_ctf.star --o
> 2mics240A140pi_autpick1 --particle_diameter 240 --angpix 1.77 --ref
> 2D_10classes.star --invert --ctf --ang 5 --lowpass 20
> --write_fom_maps --threshold 0.6 --min_distance 150
> --max_stddev_noise 1.1 '
>
> I could not work out what is wrong in the job. Could any one please
> give some comments on it?
>
> Much appreciate with your opinion.
>
> Xue
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