Hi,
please forgive me for posting once again a previous question but I
still could not find out why, when running 3D auto-refine with a
reference mask, I get the error:
"ERROR: FSC curve never drops below randomize_fsc_at. You may want to
check your mask".
I made the mask with "relion_mask_create --i run_class001.mrc --o
mask.mrc --extend_inimask 3 --lowpass 17 --angpix 0.97 --width_soft_edge
5 --ini_threshold 0.01"
so, what parameter is wrong?
Thanks a lot, Dieter
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Dieter Blaas,
Max F. Perutz Laboratories
Medical University of Vienna,
Inst. Med. Biochem., Vienna Biocenter (VBC),
Dr. Bohr Gasse 9/3,
A-1030 Vienna, Austria,
Tel: 0043 1 4277 61630,
Fax: 0043 1 4277 9616,
e-mail: [log in to unmask]
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