Does this count as an example?
grep SSBOND /a/pdb/pdb4e9m.ent
SSBOND 1 CYS A 39 CYS B 39 1555 1555 2.05
SSBOND 2 CYS C 39 CYS D 39 1555 1555 2.04
SSBOND 3 CYS E 39 CYS F 39 1555 1555 2.03
The A.U. contains three domain-swapped dimers. The cys are not on the swapped helix
but the swapping fortuitously brings the same cys in the two molecules into proximity
to make a disulfide. There are two or three other x-ray structures that show the same
domain-swapped, disulfide-clinched dimer in different packing. However an NMR structure
shows it to be monomeric in solution, based on estimated tumbling speed since
nmr restraints might not distinguish inter- from intramolecular contacts in a
domain-swapped dimer.
The cys is not conserved, and although this protein is expected to oligomerize,
the putative oligomerization domain is not included in this construct.
On 02/01/2017 10:17 AM, Eleanor Dodson wrote:
> Does anyone know of examples of these?
> I have found one - 2WQW with these SSBOND records
> 2WQW
> SSBOND 1 CYS A 206 CYS A 227 1555 6556 2.07
> SSBOND 2 CYS B 206 CYS B 227 1555 5556 2.15
>
> We seem to have one but it would have to form after crystalisation?
>
> Eleanor
>
>
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