Dear CryoEM community:
I have a job on the cluster with MPI=1, 64Gb RAM for 44.7 hour, auto
picking from two images (around 600 particles per image) in order to
optimize threshold and Dmin took 89.3hour. Here is my setting:
application='`which relion_autopick` --i 2mics_ctf.star --o
2mics240A140pi_autpick1 --particle_diameter 240 --angpix 1.77 --ref
2D_10classes.star --invert --ctf --ang 5 --lowpass 20 --write_fom_maps
--threshold 0.6 --min_distance 150 --max_stddev_noise 1.1 '
I could not work out what is wrong in the job. Could any one please give
some comments on it?
Much appreciate with your opinion.
Xue
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