On 1/19/17 3:54 PM, Panneerselvam, Saravanan wrote:
> We observed additional density around ADP that fits perfectly
> like a gamma phosphate
Hello Saravanan
At 1.4 Angstrom resolution wouldn't that suggest that you've somehow got
ATP in there ? I don't think I understand the other option - were you
proposing a ADP-O-C(O)2 arrangement to explain the density ? Surely
that has a rather different shape, considerably different scattering
power at the center of the terminal group (C vs P) and probably
different X-O bond lengths. All of these should show in the density
maps at 1.4 Å, although the bond length issue could be quite subtle.
Phil Jeffrey
Princeton
> mimicking like ATP bound state, surrounded and
> coordinated by two metal ions(resolution is 1.4A). There is a change in
> space group (from I212121 to P212121 ) and further important
> conformation changes are observed around ATP binding pocket and distant
> region. This is the only xtal we obtained in this space group, and all
> other xtals(measured 10 xtals) from the same plate belong to I212121.
>
>
>
> Thanks your help and time!
>
> Saravanan
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