Dear All,
Sorry for the little bit off topic. Is there a possibility for covalent
bond formation between beta phosphate of ADP and acetate molecule both
are coordinated by divalent metal ions? I am working on a Kinase
structure which was crystallized with ADP and in presence of 1M sodium
acetate. We observed additional density around ADP that fits perfectly
like a gamma phosphate , mimicking like ATP bound state, surrounded and
coordinated by two metal ions(resolution is 1.4A). There is a change in
space group (from I212121 to P212121 ) and further important
conformation changes are observed around ATP binding pocket and distant
region. This is the only xtal we obtained in this space group, and all
other xtals(measured 10 xtals) from the same plate belong to I212121.
Thanks your help and time!
Saravanan
----- Original Message -----
From: "CCP4BB automatic digest system" <[log in to unmask]>
To: [log in to unmask]
Sent: Sunday, 15 January, 2017 01:01:37
Subject: CCP4BB Digest - 13 Jan 2017 to 14 Jan 2017 (#2017-15)
There are 2 messages totaling 45 lines in this issue.
Topics of the day:
1. Phenix (2)
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Date: Sat, 14 Jan 2017 20:58:09 +0000
From: D Bonsor <[log in to unmask]>
Subject: Phenix
Is the phenix website down? Anyone know when it will be back up?
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Date: Sat, 14 Jan 2017 14:45:06 -0800
From: Pavel Afonine <[log in to unmask]>
Subject: Re: Phenix
See notice on Phenix mailing list that answers your question.
On Sat, Jan 14, 2017 at 12:58 PM, D Bonsor <[log in to unmask]>
wrote:
> Is the phenix website down? Anyone know when it will be back up?
>
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End of CCP4BB Digest - 13 Jan 2017 to 14 Jan 2017 (#2017-15)
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