Dear Huy,
If I understand you correctly, this is not a problem that would need
that. I think you could just use X-ray crystallography software to look
for what they call non-crystallographic symmetry (NCS) and impose that.
HTH,
Sjors
On 12/07/2016 04:21 PM, Huy Bui wrote:
> Dear Sjors, Tanmay and all,
>
> Is there anyone using Relion subtomogram averaging for 3D particles?
>
> I got a structure from Relion single particles, which contains multiple
> identical subunits (without symmetry). I want to get a higher-resolution
> from the subunit by boxing out the subunits in the map and doing 3D
> particles refinement.
>
> Is there anyway to do that in Relion using Relion subtomogram averaging
> since I got no tilt series, no missing wedge and no need for CTF correction.
>
> Best,
> Huy
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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