Sorry for my careless. I forgot to set the option to 'No'.
Thank you for your help.
Best regards,
Kotaro Tanaka
> -----Original Message-----
> From: Sjors Scheres [mailto:[log in to unmask]]
> Sent: Wednesday, November 09, 2016 7:46 PM
> To: Kotaro Tanaka <[log in to unmask]>
> Cc: [log in to unmask]
> Subject: Re: [ccpem] Auto pick filaments
>
> Hi Kotaro,
> Did you set 'Coordinates are start-end only?' to Yes? If so, change this
to No
> after autopicking.
> HTH,
> Sjors
> > Dear all,
> >
> > I performed auto-picking job for helial filaments, and after that I
> > manually checked and refined the picking results with relion gui (by
> > removing the coordinates for carbon areas, contaminations, etc...)
> >
> > Then I performed Particle extraction job, which resulted in the
> > following
> > error:
> >
> > helix.cpp::convertHelicalTubeCoordsToMetaDataTable(): Input
> > coordinates should be in pairs!
> > File: /home/people/tanaka/tanaka602/relion2-beta/src/helix.cpp line:
> > 1592
> >
> > Is this error caused by my manual refinement of the coordinates?
> >
> > I will appreciate your comments.
> >
> > Best regards,
> > Kotaro Tanaka
> >
>
>
> --
> Sjors Scheres
> MRC Laboratory of Molecular Biology
> Francis Crick Avenue, Cambridge Biomedical Campus Cambridge CB2 0QH,
> U.K.
> tel: +44 (0)1223 267061
> http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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