Hi Felipe,
During the iterations, these unfil.mrc are indeed unfiltered. However, the
behaviour you see comes from the fact they are not necessarily calculated
all the way out to Nyquist (to save computer resources). That's why it
appears as 'low-pass filtered'.
HTH,
Sjors
> Dear all,
>
> I've been recently testing the new option in Relion to use the masked
> half maps during refinement to calculate the resolution
> (--solvent_correct_fsc). During the processing the program writes many
> _unfil.mrc files which I assumed are non-filtered volumes, similar to
> the final one. However, I noticed that they have some sort of low-pass
> filter applied, which can be seen in the model.star files and the 1D
> power spectrum (see the 1D power spectrum attached). Is this intended
> and the name _unfil.mrc is just unfortunate or are these supposed to be
> really unfiltered volumes?
>
> Best
>
> Felipe
>
>
> --
> Felipe Merino
> Max Planck Institute for Molecular Physiology
> Department of Structural Biochemistry
> Otto-Hahn-Str. 11
> 44227 Dortmund
> Phone: +49 231 133 2315
>
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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