Dear Qian,
You'll need the same box size (in pixels) as the particles, and also the
pixel size (angstrom/pixel) should be the same. You can rescale or
rewindow maps using relion_image_handler.
HTH,
Sjors
On 10/11/2016 10:10 AM, Pu Qian wrote:
> Hi all,
>
> I'm going to produce an initial model for reference.mrc, but do not know
> which size I should use for it.
>
> When I pick up particle, I use 220 A as a box size. My dat has 1.06A per
> pixel. Which box size of initial model should I use, 220 or
> 220/1.06=207.5~208?
>
> Thanks for your reply in advance.
>
> Qian
>
> Sheffield University
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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